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MOS plates were imaged daily for three days using an ImageXpress Pico (Molecular Devices) cell imaging system, and cell viability was measured as described above after 72 h

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With respect to ERK, the levels of ERK1/2 and p-ERK1/2 were significantly increased in KTP mice compared to WT mice (P 0.05)

DAPI (32670), Cycloheximide (C4859), and Palmostatin B (178501) were from Sigma-Aldrich, D/D solubilizer (635054) from Takara, VECTASHIELD Mounting Medium (H-1400) from Vector Laboratories, Glutathione Sepharose 4B beads (17075601) from Cytiva, DDD85646 (13839) from Cayman Chemical, 2-bromopalmitate (sc-251714) from Santa Cruz Biotechnology, and Membrane lipid strips (P-6002) from Echelon Biosciences