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The identity of these derived cell lines was verified by confirmation of their parental origin and by validation of the intended genetic modifications, rather than by independent STR profiling

The -H2AX expression was determined by quantifying the number of foci in 50 K1 cells under each condition

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This becomes especially important when you're in a calorie deficit and using GLP-1 therapy, as appetite suppression can sometimes make it harder to eat enough protein

similar improvements have previously been observed with higher doses of subcutaneous semaglutide for obesity (Verma et al, 2022)