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Common Enzymes: Trypsin (most widely used for predictable cleavage) Chymotrypsin (targets aromatic residues) Glu-C (cleaves at acidic residues) Challenges: Incomplete digestion leading to reduced sequence coverage Overdigestion causing excessively small fragments Missed cleavage sites affecting data interpretation 3

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If a different diluent volume was used, every volume in the table above is wrong

Strengthening these muscles provides better urethral support and improves the closure mechanism that prevents leakage