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Staining controls included cells stained with appropriate isotype control antibodies (Mouse IgG2a negative control: RPE clone OX-34, Mouse IgG2b negative control: FITC, A647, Mouse IgG1 negative control: FITC, Mouse IgG3 negative control: RPE) purchased from AbD serotec, Bio-Rad laboratories GmbH, Feldkirchen, Germany

Brain glucose metabolism in the early and specific diagnosis of Alzheimers disease

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Avoiding eating within 2 hours of bedtime also helps, as lying down with a full stomach can promote acid reflux and morning queasiness

Clinical best practice calls for pinching the skin to create a fold before injection, ensuring the needle penetrates only into subcutaneous tissue