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Given the plasma concentration curve, the plasma concentration-time information for drugs can be fitted using the nonlinear least square technique, and some important pharmacokinetic data (pharmacokinetic half-life, plasma maximum, minimum blood concentration, bioavailability of drugs) are available

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In addition, we carried out similar experiments in which cells were pretreated with ITP at concentrations of 5, 10, and 20 M or insulin at concentrations of 2, 10, 50, 250, and 1250 nM, and then 5 M FITC-ITP was added, and other steps were performed as above

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The emphasis is on how reimbursement and associated cost implications may affect market penetration in the transition of oral obesity drugs from specialty care to primary care and pharmacy settings