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The current understanding, shaped largely by tirzepatide research, is more nuanced GIPR activation appears synergistic with GLP-1R activation in contexts where single-receptor work predicted a smaller signal

Microarray RNA purity and integrity were evaluated by ND-1000 Spectrophotometer (NanoDrop), Agilent 2100 Bioanalyzer (Agilent Technologies)

The inhibitory action of 1 mmol/l DON was specific to glutamine-triggered GLP-1 release, as it impaired neither basal secretion nor secretion triggered by 10 mmol/l glucose or forskolin plus IBMX (10 mol/l each)

Discontinuation rates 8.7-9.7%