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Serum markers in the rat study confirmed this: bone-specific alkaline phosphatase (BSAP) increased 31% by day 30 and remained elevated through day 90

Dohnal, V., Wu, Q

In addition, CBS protein levels have been reported to be increased following Akt activation, without affecting CBS mRNA expression (Zhu et al., 2022), which suggests that these pathways may also target CBS enzymatic activity in addition to its expression

What NAD+ does for the body: Supports the conversion of food into ATP Supports healthy mitochondrial function Helps activate sirtuins, proteins associated with cellular stress response and longevity pathways NAD+ levels naturally decline with age, which has been linked to fatigue, cognitive slowdown, and metabolic inefficiencies

These are mechanisms observed primarily in animal and cell-culture models, and they remain an active area of investigation rather than settled clinical fact