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(1997) Vitamin B12 treatment for delayed sleep phase syndrome: a multi-center double-blind study

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The tissue lysate was sequentially filtered through 70 m and 40 m cell strainers (Corning Inc., One Riverfront Plaza, NY, #352350 and #352340) to remove debris, then transferred to a new 2 mL EP tube and centrifuged at 500 g at 4 C for 5 min to obtain the pellet nuclei