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The extracted samples were separated using a Waters Acquity UPLC BEH C18 column (2.1 100 mm) on a Shimadzu Nexera UHPLC at 60 C with 0.7 mL/min flow rate, and detected using a SCIEX TripleTOF 6600 operating in full scan + MS2 positive ion mode or a SCIEX 5500 operating in MRM positive ion mode, or a Thermo TSQ Vantage operating in MRM mode

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It starts with a 0.25 mg weekly dose, which can be adjusted based on how well it works for each person