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These signals may be rapid and local or slower and systemic depending on peptide stability and receptor distribution

The positive transformants were selected on YPD plates with addition of antibiotics geneticin (G418, 4 mg/mL) or hygromycin B (HygB, 1 mg/mL) and verified by DNA sequencing after the integrated gene fragments were amplified by PCR using the corresponding primers and the genomic DNA templates

At 0.25 mg weekly, most patients notice mild changes

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