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After centrifugation for 15 min at 12,000 g , a 120 l supernatant was mixed with 100 l each of solution 1 (containing 100 mM phenol and 50 mg l 1 sodium nitroprusside) and solution 2 (comprising 0.38 M dibasic sodium phosphate, 125 mM NaOH, and 1% sodium hypochlorite with available chlorine ranging from 10 to 15%)

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The introduction of specific mutations through gene targeting should provide animal models for testing specific hypotheses regarding EDS pathogenesis