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After 24 h, cells were expanded into six-well dishes and selected in 1 g ml 1 puromycin for 3 days, then further expanded in nonselective medium

The leucine structure contains an alpha-amino group, an alpha-carboxylic acid group and a side chain isobutyl group, making it a branched-chain amino acid

Ethics declarations Ethics approval and consent to participate All procedures performed in studies involving human participants were in accordance with the ethical standards of Royan Institute and the 1964 Helsinki declaration and its later amendments or comparable ethical standards (Ethics approval number: IR.ACECR.ROYAN.REC.1397.156)

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