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Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression

Sharifi-Rad M, Anil Kumar NV, Zucca P, Varoni EM, Dini L, Panzarini E, Rajkovic J, Tsouh Fokou PV, Azzini E, Peluso I, Prakash Mishra A, Nigam M, El Rayess Y, Beyrouthy ME, Polito L, Iriti M, Martins N, Martorell M, Docea AO, Setzer WN, Calina D, Cho WC, Sharifi-Rad J

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The DM transcriptome model showed dichotomized differentially expressed genes (DEGs) response to CR, with metabolic genes upregulated and immune/inflammatory genes downregulated (15)

succinogenes was amplified through PCR using pET28a- as as the template