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Ion-sensitive polymeric systems demonstrate sol-gel transitions due to ionic stimulus in the physiological environment

Briefly, cells were grown in YPD until logarithmic phase, harvested by centrifugation at 1000 g for 3 min at room temperature, and resuspended in 200 l of One-step-transformation buffer containing 40% polyethylene glycol 3350 (Sigma Aldrich, St

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