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In a human in vivo short-term model of acute lung injury and ARDS, KGF (palifermin) pretreatment increased bronchoalveolar lavage concentrations of SP-D, a marker of type II cell proliferation, indicating its promotion of epithelial cell survival, supporting its potential benefits in ARDS

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At the time of transfection, cells were 8085% confluent and the siRNAs were transfected into STC-1 cells at a final concentration of 20 M by use of lipofectamine 2000 reagent (Invitrogen Corp., Carlsbad, CA) according to the manufacturer's instructions