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One key factor is the overexpression of ATP-binding cassette (ABC) transporters, such as ABCB1 (P-glycoprotein), which efflux cytotoxic drugs from cancer cells, thereby reducing intracellular drug concentration and efficacy (50)

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The scalability of this purification technique allows for the production of microgram, milligram, or even gram quantities of protein, catering to various experimental needs and research scales

Moreno SN, Mason RP, Docampo R

PARPs, involved in DNA repair, consume NAD+ during frequent repair events in older cells