CpG Methylase (M. Sssl) Microbial DNA Isolation The Zymo-Spin IC Fast-Spin column

The Zymo-Spin IC Fast-Spin column can be used in a microcentrifuge for the purification of DNA and/or RNA

alter cell surface and nuclear determinants

plasmid shuffling

The DNA from both reactions is then amplified using Real-Time PCR

ear punches

CpG Methylase (M. Sssl) Microbial DNA Isolation The Zymo-Spin IC Fast-Spin columnCpG Methylase completely methylates all cytosines in double stranded, non methylated, and hemimethylated DNA in a 5' CpG 3' context. The recombinant methylase is isolated from an E. coli strain that expresses the methyltransferase gene from Spiroplasma sp. strain MQ1. Reaction conditions have been optimized to maximize the processivity of the enzyme to ensure rapid, complete, and reproducible methylation of DNA for accurate DNA methylation analysis.