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Vitamin B12 is essential for nerve function, red blood cell production, and DNA synthesis

When melanocytes are hyperactivated by specific triggers UV radiation, post-inflammatory signals from acne or injury, hormonal changes (especially estrogen and progesterone in melasma), or genetic predisposition they produce excess melanin that accumulates in patches, creating dark spots, uneven tone and visible discolouration

The mitochondrial metabolism generated a considerable amount of ROS like H2O2, hydroxyl radicals, superoxide, etc

The spray voltage for both positive and negative modes was 3.5 kV and the capillary temperature was set to 320 C with a sheath gas rate of 35, aux gas of 10 and maximum spray current of 100 A