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This only needs to be done if the vial has been stored and/or exposed to low temperatures for a period of time, not prior to every injection if the vial is properly stored at room temperature

[DOI] [PubMed] [Google Scholar] 118.Russell J., Maguire S., Hunt G.E., Kesby A., Suraev A., Stuart J., Booth J., McGregor I.S

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Nat Rev Dis Primers (2017): Vitamin B12 is essential for normal nervous system function via its role in myelin synthesis and for circulatory system function through its role in red blood cell maturation in the bone marrow