For manual annotation, we obtained markers using the FindClusters function from Seurat (Supplementary Data File 1 Top 50 cell markers in each cluster ) and we observed cell-type-specific expression of a number of genes (Fig
Catalase, an antioxidant-rich enzyme, helps protect from oxidative damage and premature aging
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In contrast, in cells treated with si-PGC-1 LNP, Poly-A + RNA mainly accumulated in the nucleus, with significantly reduced signals in the cytoplasm, consistent with the results of mRNA nuclear accumulation observed through PCR experiments