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The screen was set up as follows, two concentrations of CHIR99021 (Stemgent - purity of 97% or less) were used: 3 and 4 M

16 Dysregulated mitochondrial ROS production is a feature of smokers with COPD 17,18 that is not limited to the lungs

This compound is supplied in lyophilized (freeze-dried) powder form to enhance stability for controlled laboratory research applications

Furthermore, oridonin increased the expression of the pregnane X receptor, thereby upregulating CYP3A4 and P-glycoproteinkey elements in detoxification and mucosal barrier maintenance, thus offering a dual anti-inflammatory and barrier-protective effect

We further performed doxycycline-inducible expression of oxygen-dependent NADPH oxidase (NOX) in the cytosol (TPNOX) or mitochondria (mitoTPNOX) to deplete compartmentalized NADPH, as described previously 30 , and tested the cellular sensitivity to glucose starvation (Extended Data Fig