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Lower NAD+ means reduced sirtuin activity (SIRT1, SIRT3), which further impairs mitochondrial function and fatty acid oxidation in fat cells

Second, zebrafish tolerate complex extracts such as decoctions and ethanolic preparations, preserving the potential synergistic interactions among constituents that are typically lost during early fractionation (Crawford et al., 2008)

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Interestingly, DFO dose-dependently reduced YBX1 mRNA and protein levels and AMPK protein levels but not PRKAA1 mRNA levels (Fig