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For a complex modified peptide such as eloralintidecontaining a 37-residue main chain, multiple non-standard amino acids, a methylene thioacetal bridge, site-specific Lys26 lipidation through two -Glu residues, a C20 diacid, and a C-terminal amideintact-mass MS and a single RP-HPLC purity result are not sufficient for complete structural and quality confirmation

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