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This is the same additional pathway that tirzepatide uses beyond semaglutide

Front Neurol 12:656349 Acknowledgements For the purpose of open access, this paper has been published under the creative common licence (CC-BY)

4.5 Copper Copper (Cu) plays two main roles: it promotes melanogenesis and provides physiological protection against oxidative stress by participating in the destruction of free radicals through the formation of superoxide dismutase, which catalyses the dismutation of reactive oxygen species to oxygen (O 2 ) or H 2 O 2

Coverslips were washed once in blocking buffer (1 x PBS with 2% BSA) and permeabilized with 0.5% triton X-100 for 10 min

Jiang, Y