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This primer set targets a 496 bp fragment of the BCTV coat protein 73 , under the following thermocycler conditions: initial denature of 95 C for 5 min, followed by 40 cycles of 95 C for 1 min, 58 C for 1 min, and 72 C for 1 min, and a final extension step of 72 C for 5 min