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1): 180 l of reagent A, 20 l of biomaterial, 8 l of reagent B, and 40 l of trichloroacetic acid solution

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Fluorescence microscopy Epifluorescence microscopy NAPstar or HyPer7 expressing HeLa cells were analysed with a Zeiss Axio Observer 7 inverted epifluorescence microscope and the ZEN3.2 blue edition software (Carl Zeiss AG, Oberkochen, Germany)

Adhering to safety measures during the process to reconstitute PT 141 using the guide is necessary to perform the procedure accurately and securely