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Even simple notes can start to reveal patterns, such as whether symptoms tend to be worse: After certain meals Later in the day When eating quickly or having larger portions When hydration is low For some people, symptoms dont just come and go they follow a pattern

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For whole islet staining, purified islets were fixed in 4% paraformaldehyde for 20 min and permeabilized with 0.3% Triton-X100 for 3 h at room temperature