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In another rodent experiment, synthetic TB4 was administered to models of autoimmune encephalomyelitis, markedly decreasing the number of inflammatory cells in the brains of the treated animals [39]

After incubation, the wells washed with pre-warmed RPMI 1640 (x3) and 200 l of RPMI 1640 medium added into each well, before a further 24 h incubation (20 rpm rocking, 37C)

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