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doi: 10.1002/1099-1492(200010)13:6 3.0.CO;2-C

Subsequently, chloroform was added to the supernatant, followed by centrifugation at 12,000 g for 15 min (4 C) to separate the aqueous phase

However, other types of cell death, such as typical necrosis and autophagy, also appear to be implicated in the death of melanocytes in patients with vitiligo