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The IC 25 , IC 50 , and IC 75 values for each formulation were extrapolated from the cell viability curves obtained using an Alamar Blue assay and are summarized in Table 2

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The glucagon receptor activation unique to retatrutide likely drives this effect, though the exact mechanism remains under investigation

The most robust long-term evidence comes from a 16-week study in older adults using a very closely related GHRH(1-29) analogue ([Nle27]GHRH(1-29), 10 mcg/kg subcutaneously every evening at 21:00): IGF-1 rose significantly within 2 weeks and remained elevated, lean body mass increased in men, skin thickness in both sexes, and the only adverse effect was a transient, reversible hyperlipidemia [PMID 9141536]