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The weight loss diet was determined based on individual daily energy needs using the Harris-Benedict formula, incorporating activity level and food energy consumption

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(2) Fasting that starts at a self-determined time of the day and lasts for a fixed number of hours consisting of both human activity (daytime) and inactivity (nighttime) periods (e.g., 16:8 intermittent fasting that starts at 8 pm and ends at noon)

DB, JGK, PRVJ and BT provided valuable experimental models (knockout mice and human islets) and assisted with testing them