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3 Protozoa Coccidia Two species of coccidians are the most common to infect cats, Isospora felis and Isospora rivolta (Figure 23-41 )

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BAT was chopped in 0.2% (w/v) collagenase type I solution dissolved in DMEM supplemented with penicillin (100 U/mL), streptomycin (100 g/mL), 2 mM glutamine, 10 mM HEPES, and 1.5% BSA, incubated at 37 C for 30 min in a stirring bath and filtered through a 250 m silk filter

Anesthesiology and surgical societies now recommend pausing these agents in advance so gastric function can normalize before induction