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Features of SIPS include increased senescence-associated -galactosidase (SA--Gal) activity, appearance of senescence-associated heterochromatin foci (SAHF), cell growth arrest, and increased expression of senescence-associated genes 11,34

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Degradation by the 26S proteasome is coordinated by three different types of ATP-dependent enzymes (E1, E2, and E3) that ubiquitinate the substrate and sensitize it to degradation (Scheffner et al, 1995)

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