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For cell filtering, genes detected in at least 5 cells and cells with more than 200 detected genes were selected

Holford NHG: Pharmacokinetic and pharmacodynamic principles, 2013

Not necessarily

Dehghani F, Sotoude N, Bordbar H, Panjeshahin MR, Karbalay-Doust S

They also downregulate VCAM1, IL1, and VEGF expression while upregulating VE-cadherin and ZO1 expression levels, thereby reducing inflammatory responses and endothelial cell death [48]