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After treatment, cells were immediately analyzed for apoptosis and the cell cycle using a BD FACSCelesta Cell Analyzer with an Annexin V-FITC/PI Apoptosis Detection Kit and PI/RNase Staining Buffer (BD Biosciences, Franklin Lakes, NJ, USA), respectively, according to the manufacturers instructions

DSIP (Delta Sleep-Inducing Peptide) 5 mg DSIP (Delta Sleep-Inducing Peptide) is a naturally occurring neuropeptide widely used in laboratory research to study sleep regulation, circadian rhythm modulation, and neuroendocrine function