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This was followed by haematoxylineosin staining or Nissl staining (01 % cresyl violet for 3 min), and dehydration

45 Hypothyroid patients are also more likely to develop vitamin B12 deficiency, further supporting the multifactorial influences on hair graying

26S proteasome activity assay Briefly, pulverized liver samples (20 mg aliquot) were homogenized in 26 S proteasome lysis buffer (50 mM Tris, 150 mM sodium chloride (NaCl), 1 mM ethylenediaminetetraacetic acid (EDTA), 5 mM magnesium chloride (MgCl 2) [pH 7.5]) with a hand-held Potter-Elvehjem homogenizer