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GSH Metabolism, Biosynthesis & Health Implications (PubMed, 2004) Cysteine Availability as the Rate-Limiting Factor in GSH Synthesis A widely cited review of glutathione metabolism published on PubMed documented the two-step cytosolic biosynthesis pathway in detail, confirming that GSH synthesis is regulated primarily by gamma-glutamylcysteine synthetase activity, cysteine availability, and GSH feedback inhibition

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[DOI] [PubMed] [Google Scholar] Raghu G, Berk M, Campochiaro P.A, Jaeschke H, Marenzi G, Richeldi L, Wen F.Q, Nicoletti F, Calverley P.M.A