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The total drop volume was 300 nL, consisting of 150 nL of TRF1 TRFH (31.2 mg/mL) and 150 nL of reservoir solution (1% PEG8000, 100 mM MES pH 6.0, 112 mM Mg(OAc) 2 , 1015% glycerol), whilst the reservoir volume was 35 L

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