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Template picking referenced from the cryo-EM map of the compound 2GLP-1RG s complex (EMDB code: EMD-30867) yielded 6,146,299 particle projections, which were subjected to two rounds of 2D classification to discard false positive particles or particles categorized in poorly defined classes, producing 1,497,042 particle projections

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Marguet D, Baggio L, Kobayashi T, Bernard AM, Pierres M, Nielsen PF, Ribel U, Watanabe T, Drucker DJ, and Wagtmann N

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Compare this to the indefinite treatment paradigm of pharmaceutical GLP-1 medications, where the expectation is continuous use and discontinuation typically leads to rebound