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The injection takes only seconds with a very fine needle, usually causing little to no discomfort

For peripheral nerve analysis, a 5-mm-long piece of tail 15 mm distal from the tail base was collected from each sacrificed animal, fixed in 4% formaldehyde in phosphate buffer (pH 7.4), decalcinated, and impregnated with 1% osmium tetroxide for a few days

If the protocol feels inconvenient, that's the cost of maximising outcomes

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