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Optimal clinical management of children receiving dietary therapies for epilepsy: updated recommendations of the international ketogenic diet study group

Briefly, once proteins have been spotted, the membrane strips were dried and then non-specific sites were blocked by soaking in 5% BSA in TBS-T (20 mM Tris-HCl, 150 mM NaCl, 0.05% Tween20) for 30 minutes

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The pathophysiology of endothelial dysfunction is complex, in that it is based on endothelial nitric oxide synthase uncoupling and endothelial activation following stimulation by various inflammatory mediators (molecular patterns, oxidized lipoproteins, and cytokines) (46)