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Tekland custom diet TD.170206, ENVIGO), and a second independent diet reproducing the composition of the standard 2014S diet which was supplemented with a vitamin mix without B12 (Vitamin A, Vitamin D 3 , Vitamin E, Vitamin K 3 , Vitamin B 1 , Vitamin B 2 , Niacin, Vitamin B 6 , Pantothenic Acid, Biotin, Folate and Choline) to ensure nutritional adequacy after autoclaving (Diet #2 in the text)

The mechanism involves IGF-1-mediated stimulation of osteoblast proliferation and enhanced collagen synthesis in bone matrix