n-acetylcysteine increases glutathione levels Glutathione: Master Antioxidant, Reducing Oxidative Stress, and Detoxification Can N-acetylcysteine (NAC) Supplements Increase

Liew, S.-C., & Gupta, E

Preparation Condition 4 (EDTA 4 o C deproteinization): Storage temperature affects total GSH, free GSH, and GSSG, but not the ratio of free GSH to GSSG in plasma We included EDTA-treated plasma stored at 4 o C (Preparation Condition 4) as a positive control for enzymatic degradation of glutathione which may occur during sample storage

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Oncology 18 , 11971202 (2009)