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Consequently, not only mitochondrial oxidative stress (well defined), but many other processes can be potential therapeutic targets to treat AILI

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After a further centrifugation (200 x g, 8 min, 20C, Universal 320, Andreas Hettich GmbH, Tuttlingen, Germany), PBMC were resuspended in RPMI medium supplemented with fetal bovine serum, HEPES buffer, L-glutamine, and -mercaptoethanol (all purchased from Biochrom AG, Berlin, Germany)

This occurs because small nerves beneath the skin become temporarily irritated during fat removal