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Results Combination treatment with metformin and liraglutide is more effective than single treatment to protect against palmitic acid (PA)-induced endothelial dysfunction In cultured human umbilical vein endothelial cells (HUVECs), PA induced endothelial dysfunction, characterized by increased reactive oxygen species (ROS) production, decreased NO level and reduced intracellular phosphorylated endothelial NO synthase (p-eNOS) protein level, in a concentration-dependent manner (Fig