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Indeed, chronic ROS can cause protein oxidation of methionine and cysteine residues 112,113 , which in the absence of GSH becomes permanent and might decrease irreversibly CI and CII functionality

Previous studies have confirmed that the AIF family has NADH ubiquitin oxidoreductase activity [26], so FSP1 can regenerate lipophilic groups that capture oxygen radicals through NADPH

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Cells were cultivated in RPMI medium supplemented with 10% FCS and 1% penicillin/streptomycin and incubated at 37 C and 5% CO 2 conditions