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Both toxin-induced and experimental autoimmune encephalomyelitis models show decreased BBB permeability and reduced numbers of Iba1 + macrophages following sphingosine-1-phosphate receptor 2 inactivation [95]

Sauma S, Casaccia P

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And freezing, while it preserves the unreconstituted powder, destroys the reconstituted solution by forming ice crystals that shear peptide molecules apart