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After confirmation of protein transfer using Ponceau S red staining, membranes were blocked in RotiBlock (Carl Roth GmbH & Co.KG) for 30 min

(Also worth noting: Their levels of ghrelin, a hunger hormone, significantly decreased during this period.) 3

The decrease in IGF-1 levels in T1DM happens despite the otherwise normal or elevated levels of GH, suggesting that GH-resistance is a factor