the different populations analyzed (fetuses, newborns or infants)
Cells were first stained with 50 M DCFDA or 10 M mBcl, as described above, followed by a washing step with PBS and a surface staining of 30 min on ice with combinations of the following monoclonal antibodies: PE-conjugated anti-CD10 (HI10), PerCP-conjugated anti-CD3 (SK7), APC-conjugated anti-CD19 (SJ25C1), PE-conjugated anti-CD5 (L17F12), PE-conjugated anti-CD25 (PC61), all from BD Biosciences
Therapy-induced metabolic reprogramming also plays a central role in the induction of cellular dormancy, epithelialmesenchymal transition (EMT), and the emergence of drug-tolerant persister cell populations [28]
Functional annotation highlighted genes potentially involved in AFB1 detoxification, including predicted cytochrome P450, epoxide hydrolase ( LAP2 ), glutathione-S-transferase ( URE2 ), and the ABC transporter ( YCF1 ), suggesting pathways for AFB1 activation, glutathione conjugation, and vacuolar sequestration
Moisture can promote hydrolysis (chemical breakdown due to water), and oxygen may contribute to oxidation in susceptible peptide sequences (Sigma-Aldrich, 2023b)