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2.6 Alizarin red staining (ARS) Following 14 days of osteogenic differentiation, cells were fixed with 4% paraformaldehyde for 30 min at room temperature, washed thrice with PBS, and subsequently stained with ARS staining solution (OriCell, Guangzhou, China) for 510 min
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J Intern Med 290(2):257278 Helms C, Cao L, Krueger JG, Wijsman EM, Chamian F, Gordon D, Heffernan M, Daw JAW, Robarge J, Ott J (2003) A putative RUNX1 binding site variant between SLC9A3R1 and NAT9 is associated with susceptibility to psoriasis
Studies in elderly patients with chronic fatigue found similar benefits, with ALCAR supplementation improving both physical performance and mental clarity without adverse effects